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Recent developments CMOS technology allow widefield lifetime imaging, with the ability to measure FLIM in real time, to be employed to investigate mobile samples and kinetics, as well as remote imaging.
The use of the fluorescence lifetime as a means to add contrast and extract molecular information has increased over the years. Fluorescence lifetime imaging (FLIM) using the sensitive time-correlated single-photon counting (TCSPC) technique has traditionally involved single point detection and scanning the sample. This has limited data acquisition speeds and led to the technique being thought of as slow. Developments in complementary metal oxide semiconductor (CMOS) technology has resulted in array detectors with in-pixel detection and timing to be fabricated. These recent developments allow widefield lifetime imaging, with the ability to measure FLIM in real time, to be employed to investigate mobile samples and kinetics, as well as remote imaging.
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