Quantification of Major Cannabinoids and Corresponding Acids with A-TEEM Spectroscopy

The cannabis plant (Cannabis sativa) contains naturally occurring phytocannabinoids, with D9-tetrahydrocannabinol (THC) and cannabidiol (CBD) being the most abundant. There is a need for rapid inexpensive methods to measure total THC and THC–CBD ratio (for classifying different cannabis strains).

This study, Absorbance-Transmittance Excitation Emission Matrix Method for Quantification of Major Cannabinoids and Corresponding Acids: A Rapid Alternative to Chromatography for Rapid Chemotype Discrimination of Cannabis sativa Varieties, conducted at the University of Mississippi and published in 2022 in Cannabis and Cannabinoid Research, explores the potential of simultaneous acquisition of absorbance-transmittance excitation-emission matrix data, A-TEEM™ spectroscopy, an optical technique that combines
ultraviolet-visible and fluorescence spectroscopy.